Book 25 OH Cholecalciferol ( D2 + D3 ) - Vitamin D Test

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Vitamin D is a group of essential fat-soluble vitamins, including D1, D2, and D3. It's crucial for regulating calcium and phosphorus absorption, maintaining healthy bones and teeth, and supporting the immune system.


  1. Frequent illness or infections
  2. Hair loss
  3. Muscle pain
  4. Weight gain
  5. Fatigue and tiredness
  6. Bone and back pain
  7. Depression
  8. Bone loss

What are the Normal Ranges of Vitamin D




25OH Cholecalciferol (D2+D3)


Deficiency: - Below 10; CMIA Insufficiency: - 10-30; Sufficiency: - 30-100; Hypervitaminosis:- Above 100

Safety Advice
Here is some safety Advice after detecting a Vitamin D deficiency:

  1. Consult a healthcare professional.
  2. Follow recommended supplementation.
  3. Get regular sunlight exposure (with sunscreen and during non-peak hours).
  4. Maintain a balanced diet with vitamin D-rich foods.
  5. Monitor your levels regularly.
  6. Seek personalized advice from a healthcare professional.

More Information
How to treat Vitamin D Deficiency
The treatment and prevention of vitamin D deficiency aim to achieve and maintain adequate vitamin D levels in the body.
While it is beneficial to increase the consumption of vitamin D-rich foods and spend time in sunlight, healthcare providers in India often recommend taking vitamin D supplements.
Vitamin D is available in two forms: D2 (ergocalciferol) derived from plants and D3 (cholecalciferol) derived from animals. In India, D2 supplements require a prescription, while D3 supplements are available over the counter. D3 is preferred as it is more easily absorbed by the body compared to D2.
It is important to consult with a healthcare provider in India to determine if vitamin D supplementation is necessary and to determine the appropriate dosage. They can provide guidance tailored to individual needs and specific Indian dietary patterns and lifestyles.

Certain factors can increase the risk of vitamin D deficiency, aside from medical conditions. These include:

  1. Age: Older individuals, especially those over 65 years, are more susceptible to vitamin D deficiency. Infants who rely solely on breast milk are also at risk due to its low vitamin D content.
  2. Skin color: Darker skin has a harder time producing vitamin D from sunlight compared to lighter skin, making individuals with darker skin more prone to deficiency.
  3. Mobility: People who are homebound or have limited outdoor exposure, such as those in nursing homes, face a higher risk of vitamin D deficiency. 

To prevent vitamin D deficiency, it's important to obtain sufficient vitamin D through diet or sun exposure. However, it's crucial to balance sun exposure with sunscreen use to reduce the risk of skin cancer.
Including vitamin D-rich foods in your diet, such as fatty fish, fortified dairy products, and egg yolks, can also help prevent deficiency. If you have concerns about your vitamin D levels, it's advisable to consult with a healthcare professional for personalized guidance.


  1. What is a vitamin D test?
    A vitamin D test is a blood test that measures the level of vitamin D in your body. It helps determine if you have a deficiency or if your levels are within the normal range.
  2. Why is a vitamin D test important?
    A vitamin D test is important because it helps assess your vitamin D status. Low levels of vitamin D can lead to various health issues, such as weakened bones, muscle weakness, and increased risk of certain diseases.
  3. How is the vitamin D test performed?
    The vitamin D test involves a simple blood draw. A healthcare professional will collect a small sample of your blood, usually from a vein in your arm. The sample is then sent to a laboratory for analysis. 
  4. Do I need to fast before a vitamin D test?
    No, fasting is not typically required for a vitamin D test. However, it's always best to follow any specific instructions provided by your healthcare provider or the testing facility.
  5. How long does it take to get the results?
    The turnaround time for vitamin D test results can vary depending on the laboratory. In general, it may get the result on the same day or may take a few days to a week to receive the results.
  6. What should I do if my vitamin D levels are low?
    If your vitamin D levels are low, your healthcare provider may recommend supplementation with vitamin D3, along with lifestyle changes such as increasing sun exposure and consuming foods rich in vitamin D.
  7. Can I get enough vitamin D from sunlight alone?
    While sunlight is a natural source of vitamin D, the amount of vitamin D produced in the body through sun exposure can vary based on factors such as time of day, season, geographic location, skin tone, and use of sunscreen. In some cases, supplementation or dietary sources may be necessary to maintain adequate levels.
  8. Are there any risks or side effects associated with a vitamin D test?
    A vitamin D test is a simple and safe procedure. The risks and side effects are minimal, usually limited to mild discomfort or bruising at the site of blood draw. However, it's always important to follow proper medical procedures and consult with a healthcare professional.
  9. Can vitamin D levels be too high?
    Yes, excessive vitamin D levels can be harmful. This condition, known as vitamin D toxicity or hypervitaminosis D, can lead to symptoms such as nausea, vomiting, weakness, and kidney problems. It's important to follow recommended supplementation guidelines and consult with a healthcare professional for appropriate dosing.
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Test Parameters: Alanine Amino-transferase (ALT) SGPT